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The PRDM14_Chia project in PAZAR
DescriptionThe derivation of human ES cells (hESCs) from human blastocysts represents one of the milestones in stem cell biology. The full potential of hESCs in research and clinical applications requires a detailed understanding of the genetic network that governs the unique properties of hESCs. Here, we report a genome-wide RNA interference screen to identify genes which regulate self-renewal and pluripotency properties in hESCs. Interestingly, functionally distinct complexes involved in transcriptional regulation and chromatin remodelling are among the factors identified in the screen. To understand the roles of these potential regulators of hESCs, we studied transcription factor PRDM14 to gain new insights into its functional roles in the regulation of pluripotency. We showed that PRDM14 regulates directly the expression of key pluripotency gene POU5F1 through its proximal enhancer. Genome-wide location profiling experiments revealed that PRDM14 colocalized extensively with other key transcription factors such as OCT4, NANOG and SOX2, indicating that PRDM14 is integrated into the core transcriptional regulatory network. More importantly, in a gain-of-function assay, we showed that PRDM14 is able to enhance the efficiency of reprogramming of human fibroblasts in conjunction with OCT4, SOX2 and KLF4. Altogether, our study uncovers a wealth of novel hESC regulators wherein PRDM14 exemplifies a key transcription factor required for the maintenance of hESC identity and the reacquisition of pluripotency in human somatic cells.
Statistics
Regulated genes (or markers): 4419
Regulatory sequences (genomic): 6996 Regulatory sequences (artificial): 0 Transcription factors: 1 Transcription factor profiles: 0 Annotated publications: 1 Generate GFFIf you would like to generate a current GFF file for this project immediately, click the button below to do so. The file will be made available for download in a new window. The new window may be safely closed once you have downloaded the file. Note that GFF file generation may take a significant amount of time for larger projects.Pre-generated GFF files are available in the downloads section. Public projects are checked weekly and updated files are generated if there are changes. Access project data |