The PRDM14_Chia project in PAZAR Help

Description

The derivation of human ES cells (hESCs) from human blastocysts represents one of the milestones in stem cell biology. The full potential of hESCs in research and clinical applications requires a detailed understanding of the genetic network that governs the unique properties of hESCs. Here, we report a genome-wide RNA interference screen to identify genes which regulate self-renewal and pluripotency properties in hESCs. Interestingly, functionally distinct complexes involved in transcriptional regulation and chromatin remodelling are among the factors identified in the screen. To understand the roles of these potential regulators of hESCs, we studied transcription factor PRDM14 to gain new insights into its functional roles in the regulation of pluripotency. We showed that PRDM14 regulates directly the expression of key pluripotency gene POU5F1 through its proximal enhancer. Genome-wide location profiling experiments revealed that PRDM14 colocalized extensively with other key transcription factors such as OCT4, NANOG and SOX2, indicating that PRDM14 is integrated into the core transcriptional regulatory network. More importantly, in a gain-of-function assay, we showed that PRDM14 is able to enhance the efficiency of reprogramming of human fibroblasts in conjunction with OCT4, SOX2 and KLF4. Altogether, our study uncovers a wealth of novel hESC regulators wherein PRDM14 exemplifies a key transcription factor required for the maintenance of hESC identity and the reacquisition of pluripotency in human somatic cells.

Statistics

Regulated genes (or markers): 4419
Regulatory sequences (genomic): 6996
Regulatory sequences (artificial): 0
Transcription factors: 1
Transcription factor profiles: 0
Annotated publications: 1

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Homo sapiens
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PRDM14_HUMAN ENST00000276594
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ADMC
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15688014
COMPILED
COMPUTATIONAL PREDICTION
DNASE I FOOTPRINTING
ELECTROPHORETIC MOBILITY SHIFT ASSAY (EMSA)::DIRECT GEL SHIFT
ELECTROPHORETIC MOBILITY SHIFT ASSAY (EMSA)::DIRECT GEL SHIFT WITH WESTERN BLOT DETECTION
ELECTROPHORETIC MOBILITY SHIFT ASSAY (EMSA)::GEL SHIFT COMPETITION
ELECTROPHORETIC MOBILITY SHIFT ASSAY (EMSA)::SUPERSHIFT
ELECTROPHORETIC MOBILITY SHIFT ASSAY (EMSA)::UNKNOWN
EMSA
EXPERT CURATED::LITERATURE DERIVED
GENE CO-EXPRESSION::CO-EXPRESSED GENES DETERMINED THROUGH REPORTER GENE EXPERIMENTS
IN-VIVO HUMAN CD4 ASSAY
MUTAGENESIS::EXONUCLEASE DIGESTION
MUTAGENESIS::EXONUCLEASE III DIGESTION
MUTAGENESIS::OLIGONUCLEOTIDE SYNTHESIS
MUTAGENESIS::PCR
MUTAGENESIS::RESTRICTION ENDONUCLEASE DIGESTION
MUTAGENESIS::SITE-DIRECTED
MUTAGENESIS::UNKNOWN
N/A
NA
PROTEIN BINDING ASSAY :: CHROMATIN IMMUNOPRECIPITATION WITH TAG SEQUENCING (CHIP-TS)
PROTEIN BINDING ASSAY::CHROMATIN IMMUNOPRECIPITATION (CHIP)
PROTEIN BINDING ASSAY::CHROMATIN IMMUNOPRECIPITATION WITH TAG SEQUENCING (CHIP-TS)
PROTEIN BINDING ASSAY::DNA-PROTEIN PRECIPITATION ASSAY
PROTEIN BINDING ASSAY::DNA-PROTEIN PRECIPITATION ASSAY WITH ELISA DETECTION
PROTEIN BINDING ASSAY::DNASE FOOTPRINTING ASSAY
PROTEIN BINDING ASSAY::DNASE FOOTPRINTING COMPETITION ASSAY
PROTEIN BINDING ASSAY::IN-VIVO FOOTPRINTING ASSAY
PROTEIN BINDING ASSAY::METHYLATION INTERFERENCE
PROTEIN BINDING ASSAY::METHYLATION INTERFERENCE ASSAY
PROTEIN BINDING ASSAY::UNKNOWN
PROTEIN BINDING ASSAY::WESTERN BLOT ASSAY
PROTEIN BINDING ASSAY::YEAST 1-HYBRID ASSAY
PROTEIN EXPRESSION ASSAY::ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
PROTEIN EXPRESSION ASSAY::INDIRECT IMMUNOFLUORESCENCE
PROTEIN EXPRESSION ASSAY::LUCIFERASE EXPRESSION ASSAY
PROTEIN EXPRESSION ASSAY::WESTERN BLOT ASSAY
REPORTER GENE ASSAY
REPORTER GENE ASSAY:: TRANSIENT TRANSFECTION GENE EXPRESSION ASSAY
REPORTER GENE ASSAY:: TRANSIENT TRANSFECTION GENE EXPRESSION ASSAY WITH ELISA DETECTION
REPORTER GENE ASSAY::BETA-GLOBIN EXPRESSION ASSAY
REPORTER GENE ASSAY::CHLORAMPHENICOL ACETYLTRANSFERASE (CAT) ASSAY
REPORTER GENE ASSAY::DUAL LUCIFERASE REPORTER GENE ASSAY
REPORTER GENE ASSAY::IN-VITRO VIRUS REPLICATION ASSAY
REPORTER GENE ASSAY::IN-VIVO CHLORAMPHENICOL ACETYLTRANSFERASE (CAT) ASSAY
REPORTER GENE ASSAY::IN-VIVO GENE EXPRESSION ASSAY
REPORTER GENE ASSAY::IN-VIVO GFP EXPRESSION ASSAY
REPORTER GENE ASSAY::IN-VIVO LACZ EXPRESSION ASSAY
REPORTER GENE ASSAY::IN-VIVO LUCIFERASE EXPRESSION ASSAY
REPORTER GENE ASSAY::RABBIT BETA-GLOBIN EXPRESSION ASSAY
REPORTER GENE ASSAY::SECRETED ALKALINE PHOSPHATASE (SEAP) ASSAY
REPORTER GENE ASSAY::SITE-SPECIFIC STABLE TRANSFECTION GFP ASSAY BY CRE-LOX RECOMBINATION
REPORTER GENE ASSAY::STABLE TRANSFECTION CAT ASSAY
REPORTER GENE ASSAY::STABLE TRANSFECTION DSRED ASSAY
REPORTER GENE ASSAY::STABLE TRANSFECTION LACZ ASSAY
REPORTER GENE ASSAY::STABLE TRANSFECTION LUCIFERASE ASSAY
REPORTER GENE ASSAY::STABLY INCORPORATED GFP TRANSGENE ASSAY
REPORTER GENE ASSAY::TARGETED IN-VIVO EXPRESSION ASSAY BY IN UTERO ELECTROPORATION
REPORTER GENE ASSAY::TARGETED IN-VIVO GENE EXPRESSION ASSAY BY NON-VIRAL INJECTION
REPORTER GENE ASSAY::TARGETED IN-VIVO GFP EXPRESSION ASSAY BY VIRAL INJECTION
REPORTER GENE ASSAY::TARGETED IN-VIVO LACZ EXPRESSION ASSAY BY VIRAL INJECTION
REPORTER GENE ASSAY::TRANSIENT TRANSFECTION GENE EXPRESSION ASSAY
REPORTER GENE ASSAY::TRANSIENT TRANSFECTION GFP ASSAY
REPORTER GENE ASSAY::TRANSIENT TRANSFECTION HUMAN GROWTH HORMONE ASSAY
REPORTER GENE ASSAY::TRANSIENT TRANSFECTION LACZ ASSAY
REPORTER GENE ASSAY::TRANSIENT TRANSFECTION LUCIFERASE ASSAY
REPORTER GENE ASSAY::UNKNOWN
REPORTER GENE ASSAY::VIRAL INFECTION LUCIFERASE ASSAY
RNA EXPRESSION ASSAY::IN-VITRO TRANSCRIPTION ASSAY WITH DETECTION BY PRIMER EXTENSION
RNA EXPRESSION ASSAY::NORTHERN BLOT
RNA EXPRESSION ASSAY::PRIMER EXTENSION ANALYSIS WITH POLY(A)+ RNA
RNA EXPRESSION ASSAY::REAL TIME PCR
RNA EXPRESSION ASSAY::REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR)
RNA EXPRESSION ASSAY::RNASE PROTECTION ASSAY (RPA)
RNA EXPRESSION ASSAY::WHOLE-MOUNT IN SITU HYBRIDIZATION
RNA STABILITY ASSAY::RNA SYNTHESIS BLOCKING
SELEX
SEQUENCE CONSERVATION::CO-EXPRESSED GENE CONSERVATION
SEQUENCE CONSERVATION::CONSERVATION FOUND BY ALIGNMENT
SEQUENCE CONSERVATION::ORTHOLOGOUS GENE CONSERVATION
SITE-DIRECTED MUTAGENESIS
UNKNOWN::CHROMATIN IMMUNOPRECIPITATION (CHIP)
UNKNOWN::UNKNOWN
YEAST ONE-HYBRID
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